On this research, helpful hybrid promoters had been developed for environment friendly ectopic gene expression in monocot and dicot crops, and so they maintain robust prominence in each transgenic analysis and biotech industries. This research offers with creating novel artificial promoters derived from Rice Tungro Bacilliform Virus (RTBV) and Mirabilis Mosaic Virus (MMV). Regardless of quite a few availability, there’s a extreme shortage of promoters universally appropriate for monocot and dicot crops.
Right here, eight chimeric promoter constructs had been synthesized as gBlocks gene fragments by way of area swapping and hybridization by incorporating essential domains of beforehand characterised RTBV and MMV promoters. The developed promoter constructs had been assessed for transient GUS expression in tobacco protoplast (Xanthi Brad) and agro-infiltrated tobacco, petunia, rice and pearl millet.
Protoplast expression evaluation confirmed that two promoter constructs, particularly pUPMA-RP1-MP1GUS and pUPMA-RP4-MP1GUS exhibited 3.56 and a couple of.5 instances increased actions than that of the CaMV35S promoter. We had noticed the same kind of expression patterns of those promoters in agroinfiltration-based transient research. RP1-MP1 and RP4-MP1 promoters exhibited 1.87- and 1.68-fold improve expression in transgenic tobacco crops; whereas, a 1.95-fold improve was present in RP1-MP1 transgenic rice crops compared their actions with CaMV35S promoter.
Moreover, on evaluating these promoter constructs for his or her expression within the bacterial system, pUPMA-RP1-MP1GFP was discovered to have the best GFP expression. Furthermore, the promoter assemble was additionally evaluated for its capability to specific the HMP3 gene. Biobeads of encapsulated bacterial cells expressing HMP3 gene underneath management of the pUPMA-RP4-MP1 promoter had been discovered to cut back 72.9% copper and 29.2% zinc focus from wastewater. Our outcomes had demonstrated that the developed promoter constructs might be used for translational analysis in dicot, monocot crops and bacterial methods for environment friendly gene expression.
A complete of 100 and eighty (180) diabetic sufferers and one-hundred non-diabetics (Controls) had been recruited for this research. Structured questionnaires had been administered to the consented contributors to acquire related knowledge. Sera samples obtained had been screened utilizing the HBsAg ELISA equipment; CTK Biotech, Inc, whereas the 5 panel kit-rapid diagnostic check, was used to assay for serological markers. Questionnaires had been used to acquire related data and demographic knowledge.
Hepatitis E ought to be a world public well being precedence: suggestions for bettering surveillance and prevention
Hepatitis E virus (HEV) is a vital reason behind enterically transmitted viral hepatitis and a major contributor to maternal mortality in endemic areas all over the world, but the worldwide response to this point has been restricted. HEV is a illness of poverty, and the populations experiencing the best burden of HEV-associated sickness usually are not benefitting from present interventions, together with WASH methods and immunization.
Although a vaccine exists (HEV 239, Hecolin®, Xiamen Innovax Biotech, China), it is just licensed and out there within the personal market in China and has but to be prequalified by the WHO to be used in endemic settings and outbreaks. This evaluate of the present state of HEV illness and subsequent suggestions for a coordinated public well being response are meant to information the worldwide well being neighborhood in direction of breaking the present “vicious cycle,” during which an absence of wanted knowledge prevents actions that will generate essential details about HEV as nicely
Vaccine implementation in future outbreaks, focused research assessing vaccine effectiveness and immunogenicity in endemic areas and populations, improved understanding of the worldwide burden, and enhancements in diagnostic and epidemiologic instruments are urgently wanted. Methods for implementing routine vaccination applications, bettering water, sanitation, and hygiene in endemic areas, and growing funding for analysis are additionally important.
Suggestions to Streamline and Standardize Scientific Trial Website Feasibility Assessments: An ASCO Analysis Assertion
Feasibility assessments (FAs) are essential to ascertain web site capabilities to conduct medical trials and their suitability for particular trials. Nonetheless, present FA strategies utilized by biotechnology and pharmaceutical (biotech-pharma) trial sponsors and contract analysis organizations (CROs) are pricey, inefficient, unnecessarily burdensome, and useful resource intensive. These strategies delay trial start-up, act as a barrier to web site participation, and finally cut back well timed affected person entry to medical trials and novel therapies.
An ASCO Job Pressure was convened to evaluate the precise burdens and challenges with FAs and to develop suggestions to enhance their efficiencies and effectiveness. Stakeholders (together with trial websites, biotech-pharma sponsors, and CROs) offered insights into challenges and supplied options by way of two surveys and an in-person assembly. The Job Pressure used the suggestions to formulate consensus suggestions to enhance FAs for oncology medical trials.
Three key suggestions had been recognized for utility throughout all biotech-pharma sponsored trials: (1) implement a streamlined and uniform FA course of throughout trials and sponsors; (2) reduce and standardize questions; and (3) leverage know-how to centralize FAs, facilitate communications, and cut back redundancies.
Agar, Plant Tissue Culture Tested , Type II, Dervied from Gelidium |
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| A2009-500 | GenDepot | 5Kg | EUR 1470 |
Vitamin B12 (Vit-B12) ELISA Kit |
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| E4638-100 | Biovision | EUR 874.8 | |
Vitamin B12 |
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| abx082092-1g | Abbexa | 1 g | EUR 260.4 |
Vitamine B12 |
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| abx188931-50g | Abbexa | 50 g | EUR 1095.6 |
Vitamin B12 |
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| AG115 | Unibiotest | 1 mg | EUR 627.6 |
Vitamin B12 |
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| AT115 | Unibiotest | 1mg | EUR 1641.6 |
Vitamin B12 |
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| B2063-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
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Description: Vitamin B12 |
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Vitamin B12 |
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| B2063-50 | ApexBio | 50 mg | EUR 153.6 |
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Description: Vitamin B12 |
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Vitamin B12 |
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| B2063-S | ApexBio | Evaluation Sample | EUR 97.2 |
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Description: Vitamin B12 |
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Vitamin B12 |
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| HY-B0315 | MedChemExpress | 500mg | EUR 142.8 |
Vitamin B12 |
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| GE2859-10G | Glentham Life Sciences | 10 g | EUR 228 |
Vitamin B12 |
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| GE2859-1G | Glentham Life Sciences | 1 g | EUR 84 |
Vitamin B12 |
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| GE2859-25G | Glentham Life Sciences | 25 g | EUR 399.6 |
Vitamin B12 |
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| GE2859-5G | Glentham Life Sciences | 5 g | EUR 151.2 |
Vitamin B12 |
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| TBW00737 | ChemNorm | 8XX20mg | EUR 328.8 |
BCYE AGAR (LEGIONELLA AGAR) |
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| B02-103-10kg | Alphabiosciences | 10 kg | EUR 60 |
BCYE AGAR (LEGIONELLA AGAR) |
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| B02-103-2Kg | Alphabiosciences | 2 Kg | EUR 908.4 |
BCYE AGAR (LEGIONELLA AGAR) |
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| B02-103-500g | Alphabiosciences | 500 g | EUR 364.8 |
Agar D, Purified Agar |
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| G243 | Bio Basic | 100g | EUR 97.58 |
Agar |
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| abx082160-500g | Abbexa | 500 g | EUR 260.4 |
LB AGAR - LENNOX LB AGAR |
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| L12-100-10kg | Alphabiosciences | 10 kg | EUR 991.2 |
LB AGAR - LENNOX LB AGAR |
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| L12-100-2Kg | Alphabiosciences | 2 Kg | EUR 262.8 |
LB AGAR - LENNOX LB AGAR |
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| L12-100-500g | Alphabiosciences | 500 g | EUR 115.2 |
LURIA AGAR - MILLER'S LB AGAR |
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| L12-111-10kg | Alphabiosciences | 10 kg | EUR 994.8 |
LURIA AGAR - MILLER'S LB AGAR |
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| L12-111-2Kg | Alphabiosciences | 2 Kg | EUR 262.8 |
LURIA AGAR - MILLER'S LB AGAR |
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| L12-111-500g | Alphabiosciences | 500 g | EUR 115.2 |
Vitamin B12 antibody |
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| 10-2261 | Fitzgerald | 200 ul | EUR 548.4 |
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Description: Mouse monoclonal Vitamin B12 antibody |
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Serpin B12 Antibody |
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| 47983-100ul | SAB | 100ul | EUR 302.4 |
Vitamin B12 antibody |
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| 20-VR28 | Fitzgerald | 1 ml | EUR 378 |
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Description: Rabbit polyclonal Vitamin B12 antibody |
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Vitamin B12-BSA |
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| 80-1526 | Fitzgerald | 1 mg | EUR 418.8 |
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Description: BSA conjugated Vitamin B12 Hapten |
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Vitamin B12-OVA |
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| 80-1527 | Fitzgerald | 1 mg | EUR 418.8 |
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Description: OVA conjugated Vitamin B12 Hapten |
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Vitamine B12 [BSA] |
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| DAGA-068B | Creative Diagnostics | 1mg | EUR 1014 |
Vitamine B12 [KLH] |
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| DAGA-073K | Creative Diagnostics | 1mg | EUR 1326 |
Vitamine B12 [HRP] |
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| DAGT5413-HRP | Creative Diagnostics | 1mg | EUR 1170 |
Vitamin B12 USP |
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| V004-1G | TOKU-E | 1 g | EUR 88.8 |
Vitamin B12 USP |
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| V004-5G | TOKU-E | 5 g | EUR 272.4 |
Greiner culture flasks tissue culture treated |
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| C6231-200EA | Scientific Laboratory Supplies | PK200 | EUR 189.24 |
APT AGAR |
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| A01-101-10kg | Alphabiosciences | 10 kg | EUR 1407.6 |
APT AGAR |
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| A01-101-2Kg | Alphabiosciences | 2 Kg | EUR 352.8 |
APT AGAR |
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| A01-101-500g | Alphabiosciences | 500 g | EUR 139.2 |
AGAR, BACTERIOLOGICAL |
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| A01-102-10kg | Alphabiosciences | 10 kg | EUR 2020.8 |
AGAR, BACTERIOLOGICAL |
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| A01-102-2kg | Alphabiosciences | 2kg | EUR 486 |
AGAR, BACTERIOLOGICAL |
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| A01-102-500g | Alphabiosciences | 500 g | EUR 175.2 |
AGAR,NOBLE |
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| A01-102N-10kg | Alphabiosciences | 10 kg | EUR 4692 |
AGAR,NOBLE |
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| A01-102N-2kg | Alphabiosciences | 2kg | EUR 1066.8 |
AGAR,NOBLE |
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| A01-102N-500g | Alphabiosciences | 500 g | EUR 333.6 |
ANAEROBIC AGAR |
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| A01-115-10kg | Alphabiosciences | 10 kg | EUR 3067.2 |
ANAEROBIC AGAR |
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| A01-115-2kg | Alphabiosciences | 2kg | EUR 714 |
ANAEROBIC AGAR |
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| A01-115-500g | Alphabiosciences | 500 g | EUR 237.6 |
Agar (bacteriological) |
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| A2530-2000000 | ApexBio | 2 kg | EUR 459.6 |
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Description: microbiological culture mediapasses growth performance tests with known ATCC strainsWilliams, S., Slatko, B., and McCarrey, J., Laboratory Investigations in Molecular BiologyJones and Bartlett, Sudbury, MA (2006)store dry at room temperature, CAS 9002-18-0 |
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Agar (bacteriological) |
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| A2530-500000 | ApexBio | 500 g | EUR 170.4 |
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Description: microbiological culture mediapasses growth performance tests with known ATCC strainsWilliams, S., Slatko, B., and McCarrey, J., Laboratory Investigations in Molecular BiologyJones and Bartlett, Sudbury, MA (2006)store dry at room temperature, CAS 9002-18-0 |
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Agar Bacteriological |
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| abx082171-500g | Abbexa | 500 g | EUR 693.6 |
Agar, Bacto |
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| A2007-050 | GenDepot | 500g | EUR 230.4 |
Agar, Bacto |
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| A2007-100 | GenDepot | 1Kg | EUR 360 |
Agar, Bacto |
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| A2007-250 | GenDepot | 2.5Kg | EUR 724.8 |
Agar, Bacto |
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| A2007-550 | GenDepot | 50Kg | Ask for price |
BIGGY AGAR |
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| B02-105-10kg | Alphabiosciences | 10 kg | EUR 2192.4 |
BIGGY AGAR |
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| B02-105-2Kg | Alphabiosciences | 2 Kg | EUR 523.2 |
BIGGY AGAR |
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| B02-105-500g | Alphabiosciences | 500 g | EUR 186 |
BRUCELLA AGAR |
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| B02-119-10kg | Alphabiosciences | 10 kg | EUR 1756.8 |
BRUCELLA AGAR |
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| B02-119-2kg | Alphabiosciences | 2kg | EUR 428.4 |
BRUCELLA AGAR |
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| B02-119-500g | Alphabiosciences | 500 g | EUR 160.8 |
BACTERIOLOGICAL AGAR |
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| B02-127-10kg | Alphabiosciences | 10 kg | EUR 1116 |
BACTERIOLOGICAL AGAR |
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| B02-127-2kg | Alphabiosciences | 2kg | EUR 292.8 |
BACTERIOLOGICAL AGAR |
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| B02-127-500g | Alphabiosciences | 500 g | EUR 123.6 |
DEOXYCHOLATE AGAR |
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| D04-104-10kg | Alphabiosciences | 10 kg | EUR 1582.8 |
DEOXYCHOLATE AGAR |
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| D04-104-2kg | Alphabiosciences | 2kg | EUR 391.2 |
DEOXYCHOLATE AGAR |
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| D04-104-500g | Alphabiosciences | 500 g | EUR 150 |
DRBC AGAR |
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| D04-114-10kg | Alphabiosciences | 10 kg | EUR 1465.2 |
DRBC AGAR |
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| D04-114-2Kg | Alphabiosciences | 2 Kg | EUR 366 |
DRBC AGAR |
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| D04-114-500g | Alphabiosciences | 500 g | EUR 142.8 |
CLED AGAR |
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| C03-101-10kg | Alphabiosciences | 10 kg | EUR 1782 |
CLED AGAR |
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| C03-101-2Kg | Alphabiosciences | 2 Kg | EUR 434.4 |
CLED AGAR |
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| C03-101-500g | Alphabiosciences | 500 g | EUR 162 |
CLOSTRISEL AGAR |
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| C03-110-10kg | Alphabiosciences | 10 kg | EUR 1239.6 |
CLOSTRISEL AGAR |
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| C03-110-2Kg | Alphabiosciences | 2 Kg | EUR 316.8 |
CLOSTRISEL AGAR |
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| C03-110-500g | Alphabiosciences | 500 g | EUR 129.6 |
Spectrum Agar |
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| CP044-005 | GenDepot | 500 g | Ask for price |
Spectrum Agar |
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| CP044-010 | GenDepot | 1 Kg | Ask for price |
EUGONIC AGAR |
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| E05-106-10kg | Alphabiosciences | 10 kg | Ask for price |
EUGONIC AGAR |
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| E05-106-2Kg | Alphabiosciences | 2 Kg | Ask for price |
EUGONIC AGAR |
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| E05-106-500g | Alphabiosciences | 500 g | Ask for price |
ENDO AGAR |
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| E05-110-10kg | Alphabiosciences | 10 kg | EUR 2857.2 |
ENDO AGAR |
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| E05-110-2Kg | Alphabiosciences | 2 Kg | EUR 668.4 |
ENDO AGAR |
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| E05-110-500g | Alphabiosciences | 500 g | EUR 225.6 |
MACCONKEY AGAR |
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| M13-106-10kg | Alphabiosciences | 10 kg | EUR 1124.4 |
MACCONKEY AGAR |
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| M13-106-2kg | Alphabiosciences | 2kg | EUR 291.6 |
MACCONKEY AGAR |
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| M13-106-500g | Alphabiosciences | 500 g | EUR 122.4 |
MALT AGAR |
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| M13-112-10kg | Alphabiosciences | 10 kg | EUR 60 |
MALT AGAR |
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| M13-112-2kg | Alphabiosciences | 2kg | EUR 60 |
MALT AGAR |
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| M13-112-500g | Alphabiosciences | 500 g | EUR 60 |
MYCOLOGICAL AGAR |
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| M13-138-10kg | Alphabiosciences | 10 kg | Ask for price |
MYCOLOGICAL AGAR |
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| M13-138-2kg | Alphabiosciences | 2kg | Ask for price |
MYCOLOGICAL AGAR |
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| M13-138-500g | Alphabiosciences | 500 g | Ask for price |
MYP AGAR |
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| M13-149-10kg | Alphabiosciences | 10 kg | EUR 1138.8 |
MYP AGAR |
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| M13-149-2Kg | Alphabiosciences | 2 Kg | EUR 294 |
MYP AGAR |
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| M13-149-500g | Alphabiosciences | 500 g | EUR 123.6 |
NUTRIENT AGAR |
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| N14-100-10kg | Alphabiosciences | 10 kg | EUR 1245.6 |
NUTRIENT AGAR |
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| N14-100-2kg | Alphabiosciences | 2kg | EUR 318 |
NUTRIENT AGAR |
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| N14-100-500g | Alphabiosciences | 500 g | EUR 129.6 |
NITRATE AGAR |
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| N14-107-10kg | Alphabiosciences | 10 kg | EUR 1563.6 |
NITRATE AGAR |
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| N14-107-2Kg | Alphabiosciences | 2 Kg | EUR 386.4 |
NITRATE AGAR |
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| N14-107-500g | Alphabiosciences | 500 g | EUR 148.8 |
OATMEAL AGAR |
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| O15-105-10kg | Alphabiosciences | 10 kg | EUR 1238.4 |
OATMEAL AGAR |
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| O15-105-2kg | Alphabiosciences | 2kg | EUR 315.6 |
OATMEAL AGAR |
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| O15-105-500g | Alphabiosciences | 500 g | EUR 129.6 |
GC AGAR |
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| G07-100-10kg | Alphabiosciences | 10 kg | EUR 979.2 |
GC AGAR |
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| G07-100-2kg | Alphabiosciences | 2kg | EUR 260.4 |
GC AGAR |
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| G07-100-500g | Alphabiosciences | 500 g | EUR 114 |
Agar C |
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| G1253 | Bio Basic | 100g | EUR 72.85 |
LB Agar |
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| G247 | ABM | 500 g | EUR 219.6 |
K- AGAR |
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| K11-103-10kg | Alphabiosciences | 10 kg | EUR 1656 |
K- AGAR |
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| K11-103-2kg | Alphabiosciences | 2kg | EUR 406.8 |
K- AGAR |
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| K11-103-500g | Alphabiosciences | 500 g | EUR 154.8 |
FUNGISEL AGAR |
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| F06-103-10kg | Alphabiosciences | 10 kg | EUR 3292.8 |
FUNGISEL AGAR |
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| F06-103-2Kg | Alphabiosciences | 2 Kg | EUR 763.2 |
FUNGISEL AGAR |
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| F06-103-500g | Alphabiosciences | 500 g | EUR 250.8 |
Agar A |
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| FB0010 | Bio Basic | 100g | EUR 74.62 |
Agar B |
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| FB0012 | Bio Basic | 100g | EUR 75.84 |
PHENYLALANINE AGAR |
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| P16-106-10kg | Alphabiosciences | 10 kg | EUR 1938 |
PHENYLALANINE AGAR |
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| P16-106-2kg | Alphabiosciences | 2kg | EUR 468 |
PHENYLALANINE AGAR |
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| P16-106-500g | Alphabiosciences | 500 g | EUR 170.4 |
PHENYLETHANOL AGAR |
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| P16-107-10kg | Alphabiosciences | 10 kg | EUR 1507.2 |
PHENYLETHANOL AGAR |
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| P16-107-2Kg | Alphabiosciences | 2 Kg | EUR 374.4 |
PHENYLETHANOL AGAR |
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| P16-107-500g | Alphabiosciences | 500 g | EUR 145.2 |
PALCAM AGAR |
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| P16-140-10kg | Alphabiosciences | 10 kg | EUR 1520.4 |
PALCAM AGAR |
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| P16-140-2kg | Alphabiosciences | 2kg | EUR 378 |
PALCAM AGAR |
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| P16-140-500g | Alphabiosciences | 500 g | EUR 146.4 |
SOB Agar |
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| SD7008 | Bio Basic | 250g | EUR 86.1 |
There’s an urgency to enhance the present FA course of, which is dear, inconsistent, inefficient, labor intensive, and of unsure effectiveness. All stakeholders stand to profit from implementing these suggestions, which intention to attenuate burdens and make sure that extra trial websites and sufferers have well timed entry to oncology medical trials. To have significant influence, adoption and constant execution of those suggestions throughout all trials, sponsors, CROs, and websites are important.