On this research, helpful hybrid promoters had been developed for environment friendly ectopic gene expression in monocot and dicot crops, and so they maintain robust prominence in each transgenic analysis and biotech industries. This research offers with creating novel artificial promoters derived from Rice Tungro Bacilliform Virus (RTBV) and Mirabilis Mosaic Virus (MMV). Regardless of quite a few availability, there’s a extreme shortage of promoters universally appropriate for monocot and dicot crops.
Right here, eight chimeric promoter constructs had been synthesized as gBlocks gene fragments by way of area swapping and hybridization by incorporating essential domains of beforehand characterised RTBV and MMV promoters. The developed promoter constructs had been assessed for transient GUS expression in tobacco protoplast (Xanthi Brad) and agro-infiltrated tobacco, petunia, rice and pearl millet.
Protoplast expression evaluation confirmed that two promoter constructs, particularly pUPMA-RP1-MP1GUS and pUPMA-RP4-MP1GUS exhibited 3.56 and a couple of.5 instances increased actions than that of the CaMV35S promoter. We had noticed the same kind of expression patterns of those promoters in agroinfiltration-based transient research. RP1-MP1 and RP4-MP1 promoters exhibited 1.87- and 1.68-fold improve expression in transgenic tobacco crops; whereas, a 1.95-fold improve was present in RP1-MP1 transgenic rice crops compared their actions with CaMV35S promoter.
Moreover, on evaluating these promoter constructs for his or her expression within the bacterial system, pUPMA-RP1-MP1GFP was discovered to have the best GFP expression. Furthermore, the promoter assemble was additionally evaluated for its capability to specific the HMP3 gene. Biobeads of encapsulated bacterial cells expressing HMP3 gene underneath management of the pUPMA-RP4-MP1 promoter had been discovered to cut back 72.9% copper and 29.2% zinc focus from wastewater. Our outcomes had demonstrated that the developed promoter constructs might be used for translational analysis in dicot, monocot crops and bacterial methods for environment friendly gene expression.
A complete of 100 and eighty (180) diabetic sufferers and one-hundred non-diabetics (Controls) had been recruited for this research. Structured questionnaires had been administered to the consented contributors to acquire related knowledge. Sera samples obtained had been screened utilizing the HBsAg ELISA equipment; CTK Biotech, Inc, whereas the 5 panel kit-rapid diagnostic check, was used to assay for serological markers. Questionnaires had been used to acquire related data and demographic knowledge.
Hepatitis E ought to be a world public well being precedence: suggestions for bettering surveillance and prevention
Hepatitis E virus (HEV) is a vital reason behind enterically transmitted viral hepatitis and a major contributor to maternal mortality in endemic areas all over the world, but the worldwide response to this point has been restricted. HEV is a illness of poverty, and the populations experiencing the best burden of HEV-associated sickness usually are not benefitting from present interventions, together with WASH methods and immunization.
Although a vaccine exists (HEV 239, Hecolin®, Xiamen Innovax Biotech, China), it is just licensed and out there within the personal market in China and has but to be prequalified by the WHO to be used in endemic settings and outbreaks. This evaluate of the present state of HEV illness and subsequent suggestions for a coordinated public well being response are meant to information the worldwide well being neighborhood in direction of breaking the present “vicious cycle,” during which an absence of wanted knowledge prevents actions that will generate essential details about HEV as nicely
Vaccine implementation in future outbreaks, focused research assessing vaccine effectiveness and immunogenicity in endemic areas and populations, improved understanding of the worldwide burden, and enhancements in diagnostic and epidemiologic instruments are urgently wanted. Methods for implementing routine vaccination applications, bettering water, sanitation, and hygiene in endemic areas, and growing funding for analysis are additionally important.
Suggestions to Streamline and Standardize Scientific Trial Website Feasibility Assessments: An ASCO Analysis Assertion
Feasibility assessments (FAs) are essential to ascertain web site capabilities to conduct medical trials and their suitability for particular trials. Nonetheless, present FA strategies utilized by biotechnology and pharmaceutical (biotech-pharma) trial sponsors and contract analysis organizations (CROs) are pricey, inefficient, unnecessarily burdensome, and useful resource intensive. These strategies delay trial start-up, act as a barrier to web site participation, and finally cut back well timed affected person entry to medical trials and novel therapies.
An ASCO Job Pressure was convened to evaluate the precise burdens and challenges with FAs and to develop suggestions to enhance their efficiencies and effectiveness. Stakeholders (together with trial websites, biotech-pharma sponsors, and CROs) offered insights into challenges and supplied options by way of two surveys and an in-person assembly. The Job Pressure used the suggestions to formulate consensus suggestions to enhance FAs for oncology medical trials.
Three key suggestions had been recognized for utility throughout all biotech-pharma sponsored trials: (1) implement a streamlined and uniform FA course of throughout trials and sponsors; (2) reduce and standardize questions; and (3) leverage know-how to centralize FAs, facilitate communications, and cut back redundancies.
 Agar, Plant Tissue Culture Tested , Type I , Derived from Gracilaria. |
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| A2008-100 | GenDepot | 1Kg | EUR 309 |
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Agar, Plant Tissue Culture Tested , Type I , Derived from Gracilaria. |
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| A2008-500 | GenDepot | 5Kg | EUR 1157 |
 Agar, Plant Tissue Culture Tested , Type II, Dervied from Gelidium |
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| A2009-050 | GenDepot | 500g | EUR 212 |
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Agar, Plant Tissue Culture Tested , Type II, Dervied from Gelidium |
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| A2009-500 | GenDepot | 5Kg | EUR 1225 |
 ELISA Kit) Vitamin B12 (Vit-B12) ELISA Kit |
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| E4638-100 | Biovision | EUR 729 | |
) BCYE AGAR (LEGIONELLA AGAR) |
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| B02-103-10kg | Alphabiosciences | 10 kg | EUR 50 |
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BCYE AGAR (LEGIONELLA AGAR) |
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| B02-103-2Kg | Alphabiosciences | 2 Kg | EUR 757 |
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BCYE AGAR (LEGIONELLA AGAR) |
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| B02-103-500g | Alphabiosciences | 500 g | EUR 304 |
 Agar D, Purified Agar |
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| G243 | Bio Basic | 100g | EUR 81.32 |
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 Vitamin B12 |
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| B2063-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 108 |
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Description: Vitamin B12 |
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Vitamin B12 |
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| B2063-50 | ApexBio | 50 mg | EUR 128 |
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Description: Vitamin B12 |
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Vitamin B12 |
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| B2063-S | ApexBio | Evaluation Sample | EUR 81 |
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Description: Vitamin B12 |
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Vitamin B12 |
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| AT115 | Unibiotest | 1mg | EUR 1368 |
 Vitamine B12 |
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| abx188931-50g | Abbexa | 50 g | EUR 913 |
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Vitamin B12 |
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| abx082092-1g | Abbexa | 1 g | EUR 217 |
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Vitamin B12 |
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| AG115 | Unibiotest | 1 mg | EUR 523 |
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Vitamin B12 |
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| HY-B0315 | MedChemExpress | 500mg | EUR 119 |
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Vitamin B12 |
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| GE2859-10G | Glentham Life Sciences | 10 g | EUR 190 |
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Vitamin B12 |
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| GE2859-1G | Glentham Life Sciences | 1 g | EUR 70 |
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Vitamin B12 |
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| GE2859-25G | Glentham Life Sciences | 25 g | EUR 333 |
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Vitamin B12 |
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| GE2859-5G | Glentham Life Sciences | 5 g | EUR 126 |
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Vitamin B12 |
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| TBW00737 | ChemNorm | 8XX20mg | EUR 274 |
 Agar |
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| abx082160-500g | Abbexa | 500 g | EUR 217 |
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 LB AGAR - LENNOX LB AGAR |
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| L12-100-10kg | Alphabiosciences | 10 kg | EUR 826 |
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LB AGAR - LENNOX LB AGAR |
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| L12-100-2Kg | Alphabiosciences | 2 Kg | EUR 219 |
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LB AGAR - LENNOX LB AGAR |
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| L12-100-500g | Alphabiosciences | 500 g | EUR 96 |
 LURIA AGAR - MILLER'S LB AGAR |
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| L12-111-10kg | Alphabiosciences | 10 kg | EUR 829 |
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LURIA AGAR - MILLER'S LB AGAR |
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| L12-111-2Kg | Alphabiosciences | 2 Kg | EUR 219 |
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LURIA AGAR - MILLER'S LB AGAR |
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| L12-111-500g | Alphabiosciences | 500 g | EUR 96 |
 Serpin B12 Antibody |
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| 47983-100ul | SAB | 100ul | EUR 252 |
 Vitamin B12 antibody |
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| 10-2261 | Fitzgerald | 200 ul | EUR 457 |
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Description: Mouse monoclonal Vitamin B12 antibody |
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Vitamin B12 antibody |
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| 20-VR28 | Fitzgerald | 1 ml | EUR 315 |
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Description: Rabbit polyclonal Vitamin B12 antibody |
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 Vitamine B12 [BSA] |
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| DAGA-068B | Creative Diagnostics | 1mg | EUR 845 |
 Vitamine B12 [KLH] |
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| DAGA-073K | Creative Diagnostics | 1mg | EUR 1105 |
 Vitamine B12 [HRP] |
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| DAGT5413-HRP | Creative Diagnostics | 1mg | EUR 975 |
 Vitamin B12-BSA |
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| 80-1526 | Fitzgerald | 1 mg | EUR 349 |
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Description: BSA conjugated Vitamin B12 Hapten |
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 Vitamin B12-OVA |
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| 80-1527 | Fitzgerald | 1 mg | EUR 349 |
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Description: OVA conjugated Vitamin B12 Hapten |
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 Vitamin B12 USP |
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| V004-1G | TOKU-E | 1 g | EUR 74 |
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Vitamin B12 USP |
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| V004-5G | TOKU-E | 5 g | EUR 227 |
 BIGGY AGAR |
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| B02-105-10kg | Alphabiosciences | 10 kg | EUR 1827 |
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BIGGY AGAR |
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| B02-105-2Kg | Alphabiosciences | 2 Kg | EUR 436 |
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BIGGY AGAR |
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| B02-105-500g | Alphabiosciences | 500 g | EUR 155 |
 BRUCELLA AGAR |
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| B02-119-10kg | Alphabiosciences | 10 kg | EUR 1464 |
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BRUCELLA AGAR |
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| B02-119-2kg | Alphabiosciences | 2kg | EUR 357 |
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BRUCELLA AGAR |
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| B02-119-500g | Alphabiosciences | 500 g | EUR 134 |
 BACTERIOLOGICAL AGAR |
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| B02-127-10kg | Alphabiosciences | 10 kg | EUR 930 |
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BACTERIOLOGICAL AGAR |
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| B02-127-2kg | Alphabiosciences | 2kg | EUR 244 |
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BACTERIOLOGICAL AGAR |
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| B02-127-500g | Alphabiosciences | 500 g | EUR 103 |
 DEOXYCHOLATE AGAR |
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| D04-104-10kg | Alphabiosciences | 10 kg | EUR 1319 |
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DEOXYCHOLATE AGAR |
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| D04-104-2kg | Alphabiosciences | 2kg | EUR 326 |
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DEOXYCHOLATE AGAR |
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| D04-104-500g | Alphabiosciences | 500 g | EUR 125 |
 DRBC AGAR |
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| D04-114-10kg | Alphabiosciences | 10 kg | EUR 1221 |
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DRBC AGAR |
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| D04-114-2Kg | Alphabiosciences | 2 Kg | EUR 305 |
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DRBC AGAR |
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| D04-114-500g | Alphabiosciences | 500 g | EUR 119 |
 EUGONIC AGAR |
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| E05-106-10kg | Alphabiosciences | 10 kg | Ask for price |
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EUGONIC AGAR |
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| E05-106-2Kg | Alphabiosciences | 2 Kg | Ask for price |
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EUGONIC AGAR |
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| E05-106-500g | Alphabiosciences | 500 g | Ask for price |
 ENDO AGAR |
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| E05-110-10kg | Alphabiosciences | 10 kg | EUR 2381 |
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ENDO AGAR |
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| E05-110-2Kg | Alphabiosciences | 2 Kg | EUR 557 |
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ENDO AGAR |
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| E05-110-500g | Alphabiosciences | 500 g | EUR 188 |
 APT AGAR |
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| A01-101-10kg | Alphabiosciences | 10 kg | EUR 1173 |
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APT AGAR |
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| A01-101-2Kg | Alphabiosciences | 2 Kg | EUR 294 |
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APT AGAR |
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| A01-101-500g | Alphabiosciences | 500 g | EUR 116 |
 AGAR, BACTERIOLOGICAL |
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| A01-102-10kg | Alphabiosciences | 10 kg | EUR 1684 |
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AGAR, BACTERIOLOGICAL |
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| A01-102-2kg | Alphabiosciences | 2kg | EUR 405 |
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AGAR, BACTERIOLOGICAL |
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| A01-102-500g | Alphabiosciences | 500 g | EUR 146 |
 AGAR,NOBLE |
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| A01-102N-10kg | Alphabiosciences | 10 kg | EUR 3910 |
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AGAR,NOBLE |
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| A01-102N-2kg | Alphabiosciences | 2kg | EUR 889 |
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AGAR,NOBLE |
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| A01-102N-500g | Alphabiosciences | 500 g | EUR 278 |
 ANAEROBIC AGAR |
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| A01-115-10kg | Alphabiosciences | 10 kg | EUR 2556 |
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ANAEROBIC AGAR |
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| A01-115-2kg | Alphabiosciences | 2kg | EUR 595 |
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ANAEROBIC AGAR |
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| A01-115-500g | Alphabiosciences | 500 g | EUR 198 |
 Agar Bacteriological |
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| abx082171-500g | Abbexa | 500 g | EUR 578 |
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 CLED AGAR |
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| C03-101-10kg | Alphabiosciences | 10 kg | EUR 1485 |
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CLED AGAR |
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| C03-101-2Kg | Alphabiosciences | 2 Kg | EUR 362 |
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CLED AGAR |
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| C03-101-500g | Alphabiosciences | 500 g | EUR 135 |
 CLOSTRISEL AGAR |
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| C03-110-10kg | Alphabiosciences | 10 kg | EUR 1033 |
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CLOSTRISEL AGAR |
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| C03-110-2Kg | Alphabiosciences | 2 Kg | EUR 264 |
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CLOSTRISEL AGAR |
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| C03-110-500g | Alphabiosciences | 500 g | EUR 108 |
 Spectrum Agar |
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| CP044-005 | GenDepot | 500 g | Ask for price |
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Spectrum Agar |
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| CP044-010 | GenDepot | 1 Kg | Ask for price |
 Agar, Bacto |
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| A2007-050 | GenDepot | 500g | EUR 192 |
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Agar, Bacto |
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| A2007-250 | GenDepot | 2.5Kg | EUR 604 |
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Agar, Bacto |
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| A2007-550 | GenDepot | 50Kg | Ask for price |
) Agar (bacteriological) |
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| A2530-2000000 | ApexBio | 2 kg | EUR 383 |
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Description: microbiological culture mediapasses growth performance tests with known ATCC strainsWilliams, S., Slatko, B., and McCarrey, J., Laboratory Investigations in Molecular BiologyJones and Bartlett, Sudbury, MA (2006)store dry at room temperature, CAS 9002-18-0 |
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Agar (bacteriological) |
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| A2530-500000 | ApexBio | 500 g | EUR 142 |
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Description: microbiological culture mediapasses growth performance tests with known ATCC strainsWilliams, S., Slatko, B., and McCarrey, J., Laboratory Investigations in Molecular BiologyJones and Bartlett, Sudbury, MA (2006)store dry at room temperature, CAS 9002-18-0 |
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 FUNGISEL AGAR |
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| F06-103-10kg | Alphabiosciences | 10 kg | EUR 2744 |
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FUNGISEL AGAR |
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| F06-103-2Kg | Alphabiosciences | 2 Kg | EUR 636 |
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FUNGISEL AGAR |
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| F06-103-500g | Alphabiosciences | 500 g | EUR 209 |
 GC AGAR |
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| G07-100-10kg | Alphabiosciences | 10 kg | EUR 816 |
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GC AGAR |
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| G07-100-2kg | Alphabiosciences | 2kg | EUR 217 |
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GC AGAR |
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| G07-100-500g | Alphabiosciences | 500 g | EUR 95 |
 LB Agar |
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| G247 | ABM | 500 g | EUR 183 |
 K- AGAR |
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| K11-103-10kg | Alphabiosciences | 10 kg | EUR 1380 |
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K- AGAR |
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| K11-103-2kg | Alphabiosciences | 2kg | EUR 339 |
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K- AGAR |
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| K11-103-500g | Alphabiosciences | 500 g | EUR 129 |
 PHENYLALANINE AGAR |
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| P16-106-10kg | Alphabiosciences | 10 kg | EUR 1615 |
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PHENYLALANINE AGAR |
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| P16-106-2kg | Alphabiosciences | 2kg | EUR 390 |
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PHENYLALANINE AGAR |
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| P16-106-500g | Alphabiosciences | 500 g | EUR 142 |
 PHENYLETHANOL AGAR |
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| P16-107-10kg | Alphabiosciences | 10 kg | EUR 1256 |
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PHENYLETHANOL AGAR |
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| P16-107-2Kg | Alphabiosciences | 2 Kg | EUR 312 |
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PHENYLETHANOL AGAR |
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| P16-107-500g | Alphabiosciences | 500 g | EUR 121 |
 PALCAM AGAR |
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| P16-140-10kg | Alphabiosciences | 10 kg | EUR 1267 |
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PALCAM AGAR |
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| P16-140-2kg | Alphabiosciences | 2kg | EUR 315 |
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PALCAM AGAR |
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| P16-140-500g | Alphabiosciences | 500 g | EUR 122 |
 OATMEAL AGAR |
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| O15-105-10kg | Alphabiosciences | 10 kg | EUR 1032 |
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OATMEAL AGAR |
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| O15-105-2kg | Alphabiosciences | 2kg | EUR 263 |
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OATMEAL AGAR |
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| O15-105-500g | Alphabiosciences | 500 g | EUR 108 |
 MACCONKEY AGAR |
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| M13-106-10kg | Alphabiosciences | 10 kg | EUR 937 |
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MACCONKEY AGAR |
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| M13-106-2kg | Alphabiosciences | 2kg | EUR 243 |
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MACCONKEY AGAR |
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| M13-106-500g | Alphabiosciences | 500 g | EUR 102 |
 MALT AGAR |
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| M13-112-10kg | Alphabiosciences | 10 kg | EUR 50 |
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MALT AGAR |
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| M13-112-2kg | Alphabiosciences | 2kg | EUR 50 |
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MALT AGAR |
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| M13-112-500g | Alphabiosciences | 500 g | EUR 50 |
 MYCOLOGICAL AGAR |
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| M13-138-10kg | Alphabiosciences | 10 kg | Ask for price |
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MYCOLOGICAL AGAR |
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| M13-138-2kg | Alphabiosciences | 2kg | Ask for price |
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MYCOLOGICAL AGAR |
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| M13-138-500g | Alphabiosciences | 500 g | Ask for price |
 MYP AGAR |
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| M13-149-10kg | Alphabiosciences | 10 kg | EUR 949 |
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MYP AGAR |
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| M13-149-2Kg | Alphabiosciences | 2 Kg | EUR 245 |
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MYP AGAR |
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| M13-149-500g | Alphabiosciences | 500 g | EUR 103 |
 NUTRIENT AGAR |
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| N14-100-10kg | Alphabiosciences | 10 kg | EUR 1038 |
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NUTRIENT AGAR |
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| N14-100-2kg | Alphabiosciences | 2kg | EUR 265 |
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NUTRIENT AGAR |
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| N14-100-500g | Alphabiosciences | 500 g | EUR 108 |
 NITRATE AGAR |
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| N14-107-10kg | Alphabiosciences | 10 kg | EUR 1303 |
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NITRATE AGAR |
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| N14-107-2Kg | Alphabiosciences | 2 Kg | EUR 322 |
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NITRATE AGAR |
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| N14-107-500g | Alphabiosciences | 500 g | EUR 124 |
 R2A AGAR |
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| R18-100-10kg | Alphabiosciences | 10 kg | EUR 1119 |
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R2A AGAR |
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| R18-100-2kg | Alphabiosciences | 2kg | EUR 282 |
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R2A AGAR |
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| R18-100-500g | Alphabiosciences | 500 g | EUR 113 |
There’s an urgency to enhance the present FA course of, which is dear, inconsistent, inefficient, labor intensive, and of unsure effectiveness. All stakeholders stand to profit from implementing these suggestions, which intention to attenuate burdens and make sure that extra trial websites and sufferers have well timed entry to oncology medical trials. To have significant influence, adoption and constant execution of those suggestions throughout all trials, sponsors, CROs, and websites are important.